Journal: Advanced Science

Article Title: Simultaneous Photodynamic Eradication of Tooth Biofilm and Tooth Whitening with an Aggregation‐Induced Emission Luminogen

doi: 10.1002/advs.202106071

Figure Lengend Snippet: Photodynamic antibacterial effect of DTTPB. a) Molecular structure of DTTPB. b) Schematic illustration of the simultaneous photodynamic eradication of tooth biofilm and tooth whitening processes with DTTPB. c) Evaluation of the viable state of S. mutans by using a live & dead viability/cytotoxicity assay kit (US Everbright INC.). S. mutans cells were pretreated without/with 10 µ m of DTTPB, followed by storage in dark or white‐light irradiation (36 mW cm −2 ) for 10 min. Afterwards, live & dead viability/cytotoxicity assay kit was employed to determine the viable state of S. mutans . 488 nm laser and 515–550 nm emission filter were used for the green channel, while 561 nm laser and 570–620 nm emission filter were used for the red channel. d) S. mutans survival rate evaluated by serial dilution test on BHI agar. S. mutans were treated without/with varied concentrations of DTTPB, followed by storage in dark or white‐light irradiation (36 mW cm −2 ) for 10 min. Data are presented as mean ± standard deviation with at least three replications. e) Representative images of BHI agar plates employed for quantification of S. mutans viability. Both groups were treated with 10 µ m of DTTPB. f) Morphology study of S. mutans cells. S. mutans were incubated with designated concentrations of DTTPB, followed by storage in dark or irradiating with white light (36 mW cm −2 ) for 10 min.

Article Snippet: We thus evaluated the PDT effect of DTTPB on planktonic S. mutans with a live & dead viability/cytotoxicity assay kit (US Everbright INC.).

Techniques: Cytotoxicity Assay, Irradiation, Serial Dilution, Standard Deviation, Incubation

Journal: Advanced Science

Article Title: Simultaneous Photodynamic Eradication of Tooth Biofilm and Tooth Whitening with an Aggregation‐Induced Emission Luminogen

doi: 10.1002/advs.202106071

Figure Lengend Snippet: Combination of PDT and CHX for highly effective eradication of S. mutans biofilm in in vitro oral models on glass dishes. a) Delegate 3D images of biofilms treated without/with DTTPB and white‐light irradiation. 1‐day‐old S. mutans biofilms were co‐cultured without/with 20 µ m of DTTPB and white‐light irradiation for 10 min (36 mW cm −2 ), treated without/with 0.12% CHX for 30 min, followed by staining with live & dead viability/cytotoxicity assay kit (US Everbright INC.) 488 nm laser and 515–550 nm emission filter were employed the green channel. 561 nm laser and 570–620 nm emission filter were used for the red channel. b) Quantification of survival rate according to the ratio of red/green fluorescence intensity. c,d) Quantification of (c) biofilm biomass, and (d) average thickness of live bacteria according to 5 random sights of S. mutans biofilms by COMSTAT2. Data are displayed as mean ± standard deviation.

Article Snippet: We thus evaluated the PDT effect of DTTPB on planktonic S. mutans with a live & dead viability/cytotoxicity assay kit (US Everbright INC.).

Techniques: In Vitro, Irradiation, Cell Culture, Staining, Cytotoxicity Assay, Fluorescence, Standard Deviation